Moss, moss, moss...

archaeopteryx

Gambian sidling bush
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Epiphytic 168 stack around 1.3x. I think Plagiothecium laetum but don't have the confidence level to call an identification. Partly because this individual is incompletely rehydrated, which complicates inspection of leaf margins.
P1070105 web.jpg
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archaeopteryx

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A sidewalk crack moss at 7.1x with the UPlanFL that's been amusing people lately. If one wishes to think of this as a form of high resolution photography along the image axis, it's a 1.01 GB data capture yielding 2376 frames in 15 substacks (stacked with Picolay, aligned by Hugin, post in GIMP, working size on disk is 2.03 GB). Subject size of the image is 2.44 x 1.83 mm, so the "big" stem on the right is about a millimetre in diameter.
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100% crop of the leaf cells for @Joris.
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Bushboy

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I was stunned slightly when I saw the microscope objective attached to your lens. Very clever, and I’m sure people thought it was make believe. How is it attached? Is it real?
 

archaeopteryx

Gambian sidling bush
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How is it attached?
It's attached with a 46 mm to RMS thread (0.800"-36, so named because Royal Microscopical Society boffins made it a microscope objective standard in 1858) step down ring from Joy's Optics. Most objectives use RMS so stepping rings are easy to source, though 46 mm anything tends to be trickier than 52+ mm.

Is it real?
I've not heard of anyone faking microscope objectives, though presumably it's been done. If my copy's a fake whoever did it also copied its near apochromaticity and ability to resolve submicron structures, so I think I don't care. ;) At my nominal upper limit of 9.4x the subject size of pixels in 4k videos from post focus on Panasonic's 16 MP sensors is 500 nm. At 7.1x it's 633 nm, though both are below the objective's 520 nm lateral Abbe resolution of 867 nm. Not much on that scale here, though. Counting pixels and estimating angles suggests the leaf cells above are around 7 um thick. The smaller grit particles from the sidewalk are around 15 um.

I linked the Olympus product page for the UPLFN objectives in the other thread but here it is again. Used 10x UPlanFLs are commonly available on eBay at reasonable prices, though sellers seldom specifically indicate UPLFN10X2. There are various competitive 10x infinity metallurgic objectives, the other one currently in production by Olympus being the 10x LMPlanFL (LMPLNFL).

This is the initial subslab of what was going to be a 9.4x stack of an epiphytic moss, Metaneckera menziesii. Got too "windy" to continue with the others since it doesn't take much air pressure to shift a tripod a few tens of microns.
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archaeopteryx

Gambian sidling bush
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Hi Harvey, are you sure that's moss rather than a moss gall from Diplolepis rosae or a similar wasp? Looking at full resolution on flickr I'm not seeing structures which look like the desiccated, spirally arranged leaves which would be expected of a moss colony. (Also, the mosses I know all dessicate to yellow-green, dark brown, or black rather than light brown.)

For comparison, here's a quick (handheld, not focus stacked, and low-ish magnification) snap of a couple of the branch colonies across the street from work.
P1070700 web.JPG
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Irish tribbles, fresh off our roof.
Not sure these are moss colonies either. Would need more pixels to tell if they're something else or just really pushing setae.
 
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Hi Harvey, are you sure that's moss rather than a moss gall from Diplolepis rosae or a similar wasp? Looking at full resolution on flickr I'm not seeing structures which look like the desiccated, spirally arranged leaves which would be expected of a moss colony. (Also, the mosses I know all dessicate to yellow-green, dark brown, or black rather than light brown.)

For comparison, here's a quick (handheld, not focus stacked, and low-ish magnification) snap of a couple of the branch colonies across the street from work.
View attachment 788924

Not sure these are moss colonies either. Would need more pixels to tell if they're something else or just really pushing setae.
You are correct, the growth is on a rose hip.
 

archaeopteryx

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Some wet lithophytes on concrete which proved more colourful than at first glance. 170 stack @ 1.3x.
P1070714 web.jpg
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archaeopteryx

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55 stack at 1x.
 

archaeopteryx

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P1070760C web.jpg
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169 stack around 0.4x. More about this one in another thread.
 
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archaeopteryx

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P10707816-17 162+185 10x 9.4 web.jpg
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Started out with 1912 images from 13 brackets at 9.4x but used only 347 of them. At 100% the chloroplasts are visible. Perhaps eventually I'll be able to afford a 20x or 50x objective.
P10707816-17 162+185 10x 9.4 chloroplasts.jpg
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Are those dew drops on the filaments?
Dew or captured fog. Hard to tell at that colony's particular location, though from the perspective of maintaining exohydry it's kind of an ambiguous distinction.
 

archaeopteryx

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106 stack at 1.8x.
 

archaeopteryx

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For those disinclined to watch videos this is a self-promotional link to a Panasonic G9 autofocus evaluation relevant to Ralf's animal detect thread but unrelated to this thread. The halophytic mosses Bryum capilare and Didymodon rigidulus might be present adjacent to Moss Landing in Elkhorn Slough but are not shown in the video and are absent from the local species list (Elkhorn is usually described as an estuary rather than a swamp, consistent with its limited number of trees, and Moss Landing gets its name from a wealthy man rather than a botanical source).

I suppose it's time for me to note this thread's posting recommendations again.
 

archaeopteryx

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Back on topic, a 157 stack at 3.9x.
 

archaeopteryx

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P1080002 web.jpg
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274 stack illustrating exohydric retention, 4.7x. I suspect this might be developing Rhytidiadelphus but not sure.
 

ToxicTabasco

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For those disinclined to watch videos this is a self-promotional link to a Panasonic G9 autofocus evaluation relevant to Ralf's animal detect thread but unrelated to this thread. The halophytic mosses Bryum capilare and Didymodon rigidulus might be present adjacent to Moss Landing in Elkhorn Slough but are not shown in the video and are absent from the local species list (Elkhorn is usually described as an estuary rather than a swamp, consistent with its limited number of trees, and Moss Landing gets its name from a wealthy man rather than a botanical source).

I suppose it's time for me to note this thread's posting recommendations again.
Thanks for that info. I did some online prep before going out. And the info you have is a great addition.
 
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